南京林业大学学报（自然科学版）Issue(4)：1-6,6.DOI:10.3969/j.issn.1000-2006.2015.04.001
不同美国山核桃品种花粉萌发与活力研究
A study on pollen germination and viability in different varieties of Carya illinoensis
摘要
Abstract
In order to analyze effects of different culture conditions on pollen vitality , influences of culture time,culture temperature, pH value of culture medium,sugar,boric acid and PEG-4000 concentration on pollen germination were studied by in vitro culture of four different Carya illinoensis cultivars.The results showed that culture time had extremely significant effect on the pollen germination and the pollen length of C. illinoensis. The suitable culture time was 24 h. The culture temperature,pH value,sugar boric acid and PEG-4000 concentrations in liquid medium had obvious influence on pollen viability of the four varieties.The optimum culture temperature for all varieties was 25-30℃. The weak acidic en⁃vironment was favorable for germination and growth of pollens, and the suitable pH value of liquid medium was 5.6.The pollen germination rate and pollen tube length reached their peaks when the sugar concentration was 20%-25%, the concentration of boric acid was over 0.03%;and the concentration of PEG-4000 was over 15%.关键词
美国山核桃/品种/离体培养条件/花粉萌发率/花粉管长度Key words
Carya illinoensis/variety/in vitro culture condition/pollen germination rate/pollen tube length分类
农业科技引用本文复制引用
朱雪晨,王改萍,彭方仁,吕昕..不同美国山核桃品种花粉萌发与活力研究[J].南京林业大学学报（自然科学版）,2015,(4):1-6,6.基金项目
国家林业公益性行业科研专项项目（201304711）；江苏省林业三新工程项目［Lysx2014（047）］；江苏高校优势学科建设工程资助项目 （）
