南京师大学报（自然科学版）Issue(2)：112-118,7.
利用RNA干扰技术创制高直链淀粉马铃薯新材料
Using RNAi Technology to Produce High-Amylose Potato New Material
摘要
Abstract
Amylose is extensively used in industrial applications because of it’s unique physicochemical property. Zhihong Guo inhibted expression of two potato starch branching enzyme subunit genes Sbe1 and Sbe2 by using RNA interference technology,effectively improved the amylase content in potato. To develop transgenic potato plants with high-amylose starch in tubers, the endogenesis starch branching enzymeⅠ( SBEⅠ) and Ⅱ( SBEⅡ) genes were, single or simutaneously,targeted for RNA mediated gene silencing by expression of RNA interference genes in potato. In this study,RNA interference was used for gene inhibition of the starch branching enzymeⅠ( SBEⅠ) andⅡ( SBEⅡ) genes. Three SBE-RNA interference plasmids were constructed and transformed into potato cultivar Désirée. Three transformed series were obtained and analyzed by PCR. The level of silencing of SBEⅠ and SBEⅡ in transgenic plants was investigated by analysis of apparent amylose content. The results showed that silencing of SBEⅠ or SBEⅡ alone had yielded a very low frequency of high-amylose lines, and even a reduction in amylose content in most of transgenic starches. However,production of high-amylose potato lines were be achieved by simultaneous inhibition of SBEⅠ and SBEⅡ. In this transgenic series,more than 50% of the transgenic lines showed that amylose content was higher than thire control. The highest amylose content in one line was 49. 1%,increasing 20. 6% in comparison with untransformed starch ones(28. 5%).关键词
转基因马铃薯/SBE基因/RNA干扰/直链淀粉Key words
transgenic potato/SBE gene/RNA interference/amylose分类
生物科学引用本文复制引用
朱彩云,黄翠香,于伟,王忠亚,杨金金,徐盼盼,徐春,季勤..利用RNA干扰技术创制高直链淀粉马铃薯新材料[J].南京师大学报（自然科学版）,2014,(2):112-118,7.基金项目
国家自然科学基金(30771367) （30771367）
