南京师大学报（自然科学版）Issue(3)：100-105,6.
婴儿皮肤组织成纤维细胞系的建立及生物学特性
Establishment and Characteristics of Baby Skin Explant Fibroblast
摘要
Abstract
The baby skin explants were cultured with tissue pieces method. The primary cells were purified and preserved with liquid nitrogen after being passaged to the thrid generation. Observations on morphology,dynamic growth,microbial contamination, analysis of karyotype, isoenzyme of lactate dehydrogenase and fluorescin plasmid transfection and expression were carried out. The results showed that the cell line was fibroblast and growed well. The maximum proliferation concentration was 1. 64×105/mL and poulation doubling time of cells(PDT)was 63. 12 h. The tests for bacteria,fungi,virus and mycoplasma were all negative. The chromosomes were 46 and diploid cells were dominant of 86%. The banding patterns of the isoenzyme of lactate dehydrogenase had significant difference and there were five bands in all. The exogenous plasmid could copy and express in the cell. Those showed that the cell line was established successfully,which lays the foundation of the work bases for composite artificial skin tissue engineering and provides the experimental conditions for skin diseases. So this has the important theoretical and practical significance.关键词
婴儿皮肤/成纤维细胞系/生物学特性Key words
baby skin/fibroblast cell line/characteristics分类
生物科学引用本文复制引用
王家敏,沈武玲,平玲,徐水林,赵彬,马忠仁,乔自林..婴儿皮肤组织成纤维细胞系的建立及生物学特性[J].南京师大学报（自然科学版）,2014,(3):100-105,6.基金项目
西北民族大学中青年科研基金 （）
