内蒙古农业科技Issue(4):6-9,81,5.DOI:10.3969/j.issn.1007-0907.2014.04.003
荒漠草原植物根际溶磷细菌的分离鉴定及其溶磷能力的比较
Isolation, Characterization and Phosphate-solubilizing Ability Determination of Phosphate-solubilizing Bacteria from the Rhizobacteria of Dominant Plants in Desert Steppe
摘要
Abstract
As one of the major groups of plant growth-promoting bacteria,phosphate-solubilizing bacteria play an important role in the development of green agriculture and control of desertification.The present research mainly focus on phosphate - solubilizing bacteria of farmland,classic grassland and desert ecosystem,however plant growth-promoting rhizobacteria of desert steppe has rarely been studied.The phosphate-solubilizing bacteria were isolated by the insoluble phosphate medium, and the phosphate-solubilizing ability of the isolates were detected by the potassium persulfate digestion and Mo-Sb anti colorimetric method.42 phosphate -solubilizing bacteria were isolated from the rhizosphere of four dominant plants in desert steppe of Siziwang Banner.The 42 strains were classified into 5 groups and 19 genuses.Among them,19 isolates (45.2%) belong to the Actinobacteria,5 strains (11.9%) belong to the Bacilli,16 strains (38.1%) belong to α-proteobacteria,1 strain (2.4%) belong to β- proteobacteria,and 1 strain (2.4%) belong to γ -proteobacteria.The results showed that strain GP0501,GP0506,GP0507 and GP0509 have the strongest phosphate solubilizing ability,the phosphate solubilization capacity was 77.57,65.89,58.08,68.02g/μ mL,respectively.The 4 strains have great potential of application,which all belong to Bacillus.关键词
根际促生菌/溶磷细菌/荒漠草原/分离鉴定Key words
Plant growth-promoting bacteria/Phosphate-solubilizing bacteria/Desert steppe/Isolation and identification分类
农业科技引用本文复制引用
巩瑞红,王小兵,赵吉睿,张胜男,王瑞刚,孟建宇..荒漠草原植物根际溶磷细菌的分离鉴定及其溶磷能力的比较[J].内蒙古农业科技,2014,(4):6-9,81,5.基金项目
国家自然科学基金项目(30860015);内蒙古农业大学科技创新团队项目(NDCXTD2013-3);内蒙古自治区高等学校“青年科技英才支持计划”和内蒙古农业大学科技成果转化项目资助 ()