畜牧与饲料科学Issue(1)：7-9,3.
PCR技术在猪圆环病毒2型检测中的应用
Application of PCR in the Molecular Detection of PCV-2
胡嘉彦1
作者信息
- 1. 安徽省绩溪县畜牧兽医局，安徽 绩溪 245300
- 折叠
摘要
Abstract
In present study, a PCR method was established based on the ORF2 gene of PCV-2 published in GenBank to detect the PCV-2 in clinical samples from three pig farms. The results showed that a specific band with the anticipated size of 630 bp was obtained. A total of 30 clinical samples from three pig farms were detected for the presence of PCV-2 by the established PCR method and 9 of them were PCV-2 positive, the positive rate was 30%. It was indicated that the PCR method established in this study was a fast, sentitive and efficient strategy for the molecular diagnosis of PCV-2 infection in porcine farms.关键词
猪圆环病毒2型/PCR/诊断Key words
PCV-2/PCR/diagnosis分类
农业科技引用本文复制引用
胡嘉彦..PCR技术在猪圆环病毒2型检测中的应用[J].畜牧与饲料科学,2015,(1):7-9,3.
