农业科学研究Issue(3)：34-38,5.
基于PCR-DGGE和16S rDNA克隆技术研究土壤细菌多样性的实验技术
Experiment technology of soil bacterial diversity by using PCR-DGGE and 16S rDNA clone technique
摘要
Abstract
PCR-DGGE has been widely used for measuring soil microbial diversity, but microbial diversity information is limited for identification of microbial communities. If experiment method could completely combined with16S rDNA clone technique, its limitations could reduce, and conclusions also are accurate, reliable and with good repeatability. But the technology is technical process is more complex, and technical requirements is higher. In order to afford alutary experience or lessons to many new operators, experiment technology from the extraction of DNA, PCR amplification, DGGE experiment technology, dyeing and clone technique were described respectively.关键词
PCR-DGGE/16S rDNA/克隆技术/土壤细菌多样性/实验技术Key words
PCR-DGGE/16S rDNA/clone technique/soil bacterial diversity/experiment technology分类
农业科技引用本文复制引用
刘秉儒..基于PCR-DGGE和16S rDNA克隆技术研究土壤细菌多样性的实验技术[J].农业科学研究,2014,(3):34-38,5.基金项目
国家自然科学基金项目“贺兰山土壤微生物多样性对植被垂直分异的响应机制”(41061003)；宁夏大学博士科研启动基金项目 （41061003）
