农业环境科学学报Issue(5)：862-867,6.DOI:10.11654/jaes.2014.05.006
小白菜表面粪大肠菌群的污染及其耐药性研究
Occurrence of Fecal Coliforms on Chinese Cabbages and Their Resistance to Antibiotics
摘要
Abstract
Microbial contamination of vegetables has aroused public concerns. An investigation was carried out to examine occurrence and resistance to antibiotics of fecal coliforms on fresh Chinese cabbages collected from farmers-markets and supermarkets in Jimei district of Xiamen city, China. Fecal coliforms were quantified using both MPN(most-probable-number)and PC(plate count)methods. Two treat-ments(washing or immersion in water)were used to evaluate the effect of cleaning on the abundance of fecal coliforms. The resistance of the isolated fecal coliforms to six common antibiotics(gentamicin, amikacin, penicillin, erythromycin, tetracycline, and ciprofloxacin)was eval-uated using antimicrobial susceptibility testing. The results showed that average population of fecal coliforms from the samples was 631.3 CFU·g-1 by PC method, with qualified rate of 90.9%, whereas average population of fecal coliforms was 48.2 MPN·g-1 by MPN method, with qualified rate of 87.9%. Washing and 10 min immersion reduced the number of fecal coliform bacteria to 93 CFU·g-1 and 99 CFU·g-1 from 565 CFU·g-1, respectively. The resistance the fecal coliforms to antibiotics was tetracycline(100%), penicillin(100%), and erythromycin (85%). However, the coliforms had no resistance to gentamicin, amikacin, and ciprofloxacin. Our results suggest that cleaning treatments can significantly reduce the number of fecal coliforms and the fecal coliforms have resistance to common antibiotics.关键词
MPN/平板计数/药敏试验/耐药性/粪大肠菌群Key words
MPN/CFU/antimicrobial susceptibility testing/resistance/fecal coliform分类
资源环境引用本文复制引用
李琼,李雅颖,姚槐应,葛超荣..小白菜表面粪大肠菌群的污染及其耐药性研究[J].农业环境科学学报,2014,(5):862-867,6.基金项目
国家自然科学基金项目（81101283）；宁波市自然科学基金 （）
