农业环境科学学报Issue(5)：882-889,8.DOI:10.11654/jaes.2014.05.008
苯胺降解菌R hodococcus sp. E2的分离与苯胺氧化酶基因簇的初步鉴定
Isolation of Aniline-degrading Bacterium Rhodococcus sp. E2 and Characterization of Aniline Dioxygenase Gene Cluster
摘要
Abstract
Aniline and its derivatives have become environmental pollutants. The existing aniline-degrading bacteria have narrow substrate spectrum toward aniline derivatives and can degrade parts of these compounds. Here we isolated an aniline-degrading bacterium E2 from activated sludge originated from an insecticide factory, and revealed its molecular nature. The strain E2 was identified as Rhodococcus sp. based on morphological, physiological and biochemical properties, and homology analysis of 16S rDNA sequence. The optimum temperature and pH value for the degradation by the strain E2 were 30℃and 7.0, respectively. The highest aniline concentration at which the strain E2 could degrade was 800 mg·L-1. It could degrade aniline, o-Toluidine, p-Toluidine and 2,5-dichloroaniline. Aniline-degrading gene cluster was cloned from the strain E2 by amplifying the highly conserved fragment and chromosome walking. This cluster was significantly different from the current ones in composition, arrangement and homology of genes. It would be a valuable material for investigating the molecular mechanisms of aniline degradation.关键词
苯胺/Rhodococcus sp. E2/基因簇Key words
aniline/Rhodococcus sp. E2/gene cluster分类
农业科技引用本文复制引用
李云翔,陈雪婷,周潮洋,裴涛,刘永闯,洪青,闫新,李顺鹏..苯胺降解菌R hodococcus sp. E2的分离与苯胺氧化酶基因簇的初步鉴定[J].农业环境科学学报,2014,(5):882-889,8.基金项目
国家基础科学人才培养基金 （）
