农业环境科学学报Issue(10)：1890-1896,7.DOI:10.11654/jaes.2014.10.003
铜胁迫和间作对玉米抗氧化酶活性及丙二醛含量的影响
Effects of Copper Stresses and Intercropping on Antioxidant Enzyme Activities and Malondialdehyde Contents in Maize
摘要
Abstract
With industrial development, the area of arable land contaminated by heavy metals is steadily increasing around the world, espe-cially in developing countries. Intercropping has shown the potential to phytoremediate heavy metal polluted soils. A pot experiment was conducted to explore the antioxidant responses of maize to copper(Cu)pollution in red soil under maize-pea intercropping. Five Cu concentrations(0, 100, 200, 400, 600 mg·kg -1)and two cropping patterns(maize monoculture and maize-pea intercropping)were de-signed. Plant biomass, Cu contents, antioxidant enzyme activities(SOD, CAT, POD)and MDA contents of maize were measured. Com-pared with those in the monoculture, the aboveground and underground dry biomass of maize in high Cu2+ concentration(600 mg·kg-1)were increased by 20% and 36.6%, respectively, under the intercropping system. However, Cu contents in the aboveground were reduced by 86.81%, 44.57%, 22.01% and 86.81%, but root Cu increased by 78.89%, 24.79%, 35.29% and 24.79% in 100, 200, 400 mg·kg-1 and 600 mg·kg-1 treatments, respectively, under the intercropping system, compared with the maize monoculture. Significant difference in plant Cu was present between intercropping and monoculture. The SOD, POD, CAT activities and MDA content in leaves and roots of maize all in-creased with increasing Cu2+ concentrations. But Cu2+ addition at 600 mg·kg-1 caused CAT activity decrease. Compared to the monoculture, SOD activity under maize-pea intercropping increased by 48.07%~117.27% in roots and 11.30%~46.90% in leaves. Cropping patterns had no significant effect on POD activity under the same Cu2+ stress. In 0~400 mg·kg-1 Cu stresses, CAT activities in maize leaves were signifi-cantly increased by 71.37%, 140.40%, 229.80% and 161.75% respectively under the intercropping over the monoculture, whereas no sig-nificant difference in the CAT activities in roots was observed between two cropping systems. The intercropping practice reduced MDA con-tents in maize roots by 26.13%~64.53%; while intercropping caused left MDA decline only at 100 mg Cu·kg-1 and 200 mg Cu·kg-1 but in-creased at 400 mg Cu·kg-1 and 600 mg Cu·kg-1, as compared with the monoculture. In summary, intercropping could improve antioxidant enzymatic system in maize roots and leaves and thus alleviate Cu toxicity under Cu2+ stresses.关键词
铜/抗氧化酶/丙二醛/间作Key words
copper/antioxidases/malondialdehyde/intercropping分类
资源环境引用本文复制引用
王晓维,黄国勤,徐健程,聂亚平,万进荣,杨潇一,杨文亭..铜胁迫和间作对玉米抗氧化酶活性及丙二醛含量的影响[J].农业环境科学学报,2014,(10):1890-1896,7.基金项目
国家自然科学基金项目（31360108）；江西省大学生创新训练计划项目 （）
