农业环境科学学报Issue(11)：2155-2163,9.DOI:10.11654/jaes.2014.11.013
双价转基因抗虫棉花对土壤氨氧化细菌和氨氧化古菌群落结构及丰度的影响
Community Structure and Abundance of Soil Ammonia-oxidizing Bacteria and Ammonia-oxidizing Archea as Influenced by Insect-resistant Bivalent Transgenic Cotton
摘要
Abstract
The cultivation area of genetically modified(GM)crops has expanded significantly in recent years. However, concerns have been raised over impacts of GM crops on soil ecosystem, especially soil microorganisms. Here, we examined the influence of insect-resistant biva-lent transgenic cotton on soil ammonia-oxidizing bacteria(AOB)and ammonia-oxidizing archaea(AOA). Terminal restriction fragment length polymorphism(T-RFLP)was used to evaluate community structure change and qPCR to detect abundance difference. Compared to the control, the population of dominant AOB and AOA did not show significant difference in insect-resistant bivalent transgenic cotton soil, with no changes over the growth season though the ratio of each dominant species population varied in different varieties and at different growth stages of cotton. The Shannon index and Evenness index of AOB had no significant difference between the transgenic cotton soil and its control during the whole growth period. The Shannon index of AOA had similar results, but Evenness index of AOA was significantly low-er in the transgenic cotton soil than in the control at the seedling stage(P<0.05), with no difference found at the other growth stages. The abundance of AOB in the transgenic cotton soil was higher at the blooming stage, whereas lower at the other stages, as compared with the control soil. However, AOA had lower abundance in the GM cotton than in the control soil throughout the growing season. Therefore, the in-sect-resistant bivalent transgenic cotton had no significant impact on the community structure of soil AOB and AOA, but reduced the abun-dance of AOB and AOA, suggesting potential impacts of transgenic cotton on soil ammonia-oxidizing microorganisms.关键词
双价转基因抗虫棉花/氨氧化细菌/氨氧化古菌/栽原砸云蕴孕/实时定量PCRKey words
insect-resistant bivalent transgenic cotton/ammonia-oxidizing bacteria/ammonia-oxidizing archaea/T-RFLP/quantitative real-time PCR分类
农业科技引用本文复制引用
吴元凤,李刚,修伟明,冀国桢,宋晓龙,赵建宁,杨殿林..双价转基因抗虫棉花对土壤氨氧化细菌和氨氧化古菌群落结构及丰度的影响[J].农业环境科学学报,2014,(11):2155-2163,9.基金项目
国家自然科学基金（31200424）；中央级公益性科研院所基本科研业务费专项资金 （）
