农业环境科学学报Issue(3)：570-577,8.DOI:10.11654/jaes.2015.03.021
接种菌剂对牛粪堆肥反硝化细菌群落的影响
Effect of Microbial Inoculum on Denitrifying Bacterial Communities in Cow Manure Compost
摘要
Abstract
Aerobic composting is an effective method to dispose solid organic wastes. In this study, cow manure with rice straw was used to investigate the effect of lignocelluloses-degrading microbial inoculum on denitrifying bacterial communities. Composting maturity was as-sessed by temperature, pH, C/N, seed germination index(GI), NH﹢4-N and NO-3-N contents. Both natural and microbes-inoculated com-posts were completely mature, with the inoculated compost more mature than natural one. Microbial inoculation decreased denitrifying bac-terial population, but increased the diversity of nosZ-denitrifying bacterial community. The similarity of natural compost samples ranged from 50%to 75%, while that among microbe-inoculated samples was between 27%and 76%, indicating an accelerated succession of deni-trifying bacterial communities by inoculated microbes. NosZ-denitrifying bacteria in compost includedγ-proteobacteria,β-proteobacteria, uncultured bacteria andα-proteobacteria. Microbes-inoculated compost contained some denitrifying bacteria taxas that were not present in the natural compost. These taxas were Halomonas, Azospirillum, Paracoccus, Herbaspirillum and Alcaligenes. Among them, the final deni-trifying product of Alcaligene and Paracoccus was N2, thus decreasing N2O emissions.关键词
自然堆肥/接种菌剂堆肥/腐熟/反硝化细菌数量/反硝化细菌多样性Key words
natural compost/microbes-inoculated compost/maturity/denitrifying bacterial population/denitrifying bacterial diversity分类
资源环境引用本文复制引用
徐莹莹,许修宏,任广明,孙瑜,李洪涛..接种菌剂对牛粪堆肥反硝化细菌群落的影响[J].农业环境科学学报,2015,(3):570-577,8.基金项目
国家自然科学基金 （）
