农业环境科学学报Issue(4)：672-678,7.DOI:10.11654/jaes.2015.04.010
一株荧蒽降解菌Herbaspirillum chlorophenolicum strain FA1的固定化及其优化
Immobilization of Fluoranthene-Degrading Herbaspirillum chlorophenolicum strain FA1 and Its Optimization
摘要
Abstract
Microbial remediation of polycyclic aromatic hydrocarbons(PAHs), a group of persistent toxic contaminants that are widespread in terrestrial and aquatic environments, has received increasing interests. A fluoranthene-degrading strain FA1 previously isolated from acti-vated sludge has showed capability to use fluoranthene as sole carbon and energy sources and to degrade fluoranthene in the presence of in-digenous flora. In this study, immobilization of fluoranthene-degrading strain FA1 by various matrices and its effects on fluoranthene degra-dation in soil-water system were investigated. Results showed that the strain FA1 immobilized on polyvinyl alcohol(PVA)-diatomite matrix prepared by chemical method(boric acid)exhibited the highest degradation of fluoranthene, with a 97.74% removal of fluoranthene from soil-water system after 25 d. PVA-diatomite and PVA-activated carbon matrices obtained by chemical method showed better effectiveness than those obtained by freezing and thawing method. The optimal immobilization conditions of PVA- diatomite matrix(chemical method) were 9.6%of cell density, 11.2%of PVA, 4.5%of diatomite and 4.0 mm of bead size. Under these conditions, 99.46%of fluoranthene was removed from the soil-water system after 20 d.关键词
荧蒽/草螺菌属细菌/生物降解/固定化/响应曲面法Key words
fluoranthene/Herbaspirillum chlorophenolicum strain FA1/biodegradation/immobilization/response surface methodology分类
资源环境引用本文复制引用
徐红霞,李宵慧,孙媛媛,施小清,吴吉春..一株荧蒽降解菌Herbaspirillum chlorophenolicum strain FA1的固定化及其优化[J].农业环境科学学报,2015,(4):672-678,7.基金项目
国家自然科学基金（41102148，41030746）；高等学校博士学科点专项科研基金资助课题 （）
