农业环境科学学报Issue(6)：1041-1046,6.DOI:10.11654/jaes.2015.06.004
高/低Cd累积苋菜品种金属累积特性与关键转运基因表达的关系
Metal Accumulation and Expressions of Root Two Key Transporter Genes, HMA4 and IRT1, of High/Low Cd-Accumulating Amaranth Cultivars
摘要
Abstract
Metal accumulation in plants may be controlled by their transporter gene expressions. Here a pot experiment was conducted to in-vestigate the differences of metal uptake by edible amaranth cultivars in relation to the expressions of two key transporter genes, HMA4 and IRT1, in roots using garden soil polluted with heavy metals. The expressions of genes IRT1 and HMA4 in root were determined by semi-quantitative PCR method. Cultivars Baiguxiaoyuanye and Zhengtaixiaoyuanye accumulated(0.116±0.007)mg·kg-1 and(0.146±0.002)mg· kg-1(fresh weight)Cd in shoot, but Taiwanbai and Hongliuye contained(0.210±0.005)mg·kg-1 and(0.349±0.026)mg·kg-1(fresh weight) Cd in shoot, respectively. Therefore, the later two are high-Cd accumulating cultivars. Cadmium accumulation in root protoplasts was posi-tively correlated with Fe and Pb(P<0.01). Concentrations of Cd, Fe and Pb in and the expressions of gene IRT1 in root protoplasts of high-Cd accumulating cultivar Hongliuye were significantly higher than those in low-Cd accumulating cultivar Baiguxiaoyuanye. This implied that higher expression of gene IRT1 in root might contribute to Fe uptake by root, as well as Cd and Pb uptake. Cadmium accumulation in shoot was significantly positively correlated with Zn and Fe(P<0.05)and extremely significantly with Pb, Ca and Mn(P<0.01). Concentrations of Cd, Ca, Fe, Mn and Pb in shoot of high-Cd accumulating cultivar Hongliuye and its expression of gene HMA4 were significantly higher than those in low-Cd accumulating cultivar Baiguxiaoyuanye. This suggests that the higher expression of gene HMA4 in root may facilitate Cd and other metals transport from roots to shoots.关键词
苋菜/镉累积/IRT1/HMA4/品种差异Key words
edible amaranth/Cd accumulation/IRT1/HMA4/cultivar difference分类
资源环境引用本文复制引用
陈艳芳,李取生,何宝燕,梅秀芹,雷永康,徐智敏,周丽珍..高/低Cd累积苋菜品种金属累积特性与关键转运基因表达的关系[J].农业环境科学学报,2015,(6):1041-1046,6.基金项目
国家自然科学基金项目（41371321）；中央高校基本科研基金 （）
