首页|期刊导航|农业环境科学学报|多环芳烃降解菌的细胞融合及降解性能研究

多环芳烃降解菌的细胞融合及降解性能研究OA北大核心CSCDCSTPCD

Cell Fusion of PAHs-degrading Bacteria and Its Degradation Ability

中文摘要英文摘要

以菲降解菌———鞘氨醇单胞菌(Sphingomonas sp.)GY2B和芘降解菌———假单胞菌(Sphingomonas sp.)GP3A为研究对象,对两株菌进行融合前的抗药性标记筛选,融合后的菌株通过形态学及分子生物学进行分析鉴定,并测定其对菲和芘的降解效果。结果表明,筛选出GY2B的遗传标记为哌拉西林抗性(80μg·mL-1),GP3A的遗传标记为头孢他啶抗性(80μg·mL-1)或红霉素抗性(100~150μg·mL-1)。通过菲和芘的初…查看全部>>

In the present study, a phenethrene-degradation strain(Sphingomonas sp.)GY2B and a pyrene-degradation strain(Pseudomonas sp.)GP3A were used to obtain a fusant capable of degrading PAHs. The antibiotic resistance test was used to screen the genetic markers. Morphology and molecular biology were employed to identify fusants. Results showed that the genetic markers of Sphingomonas sp. GY2B was piperacillin(80μg·mL-1)-resistant and Pseudomonas sp. GP3A was cefta…查看全部>>

卢静;侯彬;郭楚玲

中北大学化工与环境学院,太原 030051中北大学化工与环境学院,太原 030051华南理工大学环境与能源学院,广州 510006

资源环境

融合菌遗传标记多环芳烃

phenanthrenepyrenefusant straingenetic markerPAHs

《农业环境科学学报》 2015 (6)

1134-1141,8

国家自然科学基金青年基金(41401355,41101291);山西省青年科技研究基金(2014021034-2,2015021053)

10.11654/jaes.2015.06.017

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