农业资源与环境学报Issue(3)：237-245,9.DOI:10.13254/j.jare.2014.0026
氮素和水分对贝加尔针茅草原土壤酶活性和微生物量碳氮的影响
Effects of Nitrogen and Water on Soil Enzyme Activity and Soil Microbial Biomass in Stipa baicalensis Steppe, Inner Mongolia of North China
摘要
Abstract
In this paper, eight nitrogen treatments were applied at 0 g·m-2(N0), 1.5 g·m-2(N15), 3.0 g·m-2(N30), 5.0 g·m-2(N50), 10.0 g· m-2(N100), 15.0 g·m-2(N150), 20.0 g·m-2(N200), 30.0 g·m-2(N300)as NH4NO3 and adding water to simulate summer rainfall of 100 mm, the interactive experiment was set to explore the effects of nitrogen and water addition in Stipa baicalensis steppe on soil nutrients, enzyme activities and soil microbial biomass. The results showed that the nitrogen and water addition changed soil physico-chemical factors obvious-ly, the content of soil total organic carbon, total nitrogen, nitrate nitrogen and ammonium nitrogen increased along with the increasing of ap-plication rate of nitrogen, on the contrary, the soil pH value had decreasing trend. Appropriate application of nitrogen could enhance the ac-tivity of urease and catalase but decreased the activity of polyphenol oxidase. Nitrogen and water addition had significant effect on soil micro-bial biomass C and N. Higher level of N fertilizer significantly reduced microbial biomass C, and the microbial biomass N was on the rise with the application rate of nitrogen. The addition of water could slow the inhibition of nitrogen to microorganism and increase the microbial biomass C and N. A closed relationship existed in soil nutrient, activities of soil enzyme and soil microbial biomass C and N. The significantly positive correlation existed between total N, organic C, nitrate N and catalase, significantly negative correlation between nitrate N, ammonium N, total N and polyphenol oxidase. Microbial biomass N was significantly positive correlated with total N, nitrate N, ammonium N, catalase, phosphatase, and was negative correlated with polyphenol oxidase. Microbial biomass C was significantly positive correlated with polyphenol oxidase, and was negative correlated with catalase.关键词
贝加尔针茅草原/氮素添加/水分添加/土壤理化性质/酶活性/微生物量碳/微生物量氮Key words
Stipa baicalensis steppe/nitrogen addition/water addition/soil physico-chemical properties/enzyme activities/microbial biomass C/microbial biomass N分类
农业科技引用本文复制引用
王杰,李刚,修伟明,宋晓龙,赵建宁,杨殿林..氮素和水分对贝加尔针茅草原土壤酶活性和微生物量碳氮的影响[J].农业资源与环境学报,2014,(3):237-245,9.基金项目
国家自然科学基金项目（31170435,31000242）；“十二五”国家科技计划项目 （）
