农业生物技术学报Issue(11)：1447-1454,8.DOI:10.3969/j.issn.1674-7968.2014.11.016
橘小实蝇蛋白饵剂中氨基酸的HPLC分析及生物测定
HPLC Analysis and Biological Test of Amino Acids in Protein Bait for Bactrocera dorsalis (Hendel)
摘要
Abstract
This article mainly compares the similarities and differences between Prima produced by Malaysia Pupuk Alam company and protein bait for Bactrocera dorsalis (Hendel) developed by our research institute. The biological test was used to compare attractive effects, and High Performance Liquid Chromatography with Precolumn Derivatization (AccQ · Tag) was used to analyse the kinds and contents of amino acids. The results showed the attractive effects of Prima and protein bait were similar, and the two protein baits all contained 17 kinds of free amino acids and 18 total amino acids. Prima contained 52.847 6 mg/mL of total amino acids, 5.736 4 mg/mL of free amino acid, and 47.111 2 mg/mL of peptides. The protein bait was consisted of 65.624 3 mg of total amino acids, 6.301 0 mg of free amino acid, 59.323 3 mg of peptides in one milliliter. Besides, the attractive effect of hydrolyzed protein was stronger than before. The results can provide a basis for monitoring protein bait quality on the industrial production.关键词
橘小实蝇/蛋白饵剂/游离氨基酸/总氨基酸/高效液相色谱Key words
Bactrocera dorsalis(Hendel)/Protein bait/Free amino acid/Polypeptide amino acid/Chromatograph引用本文复制引用
韩英,王波,许晨,许建中,黄居昌,季清娥,杨建全,陈家骅..橘小实蝇蛋白饵剂中氨基酸的HPLC分析及生物测定[J].农业生物技术学报,2014,(11):1447-1454,8.基金项目
国家自然科学基金面上项目(No.11175046) （No.11175046）
