农药学学报Issue(5)：508-516,9.DOI:10.3969/j.issn.1008-7303.2014.05.03
鲫鱼γ-氨基丁酸受体β3亚基基因克隆及其分析
Cloning and analysis of γ-aminobutyric acid receptorβ3 submit of Carassius auratus
摘要
Abstract
γ-aminobutyric acid receptor ( GABAR ) mainly exist in the central nervous system of vertebrates and invertebrates ( CNS ) , it is the important targets of pesticides such as fipronil, abamectin, endosulfan and lindane. In order to clarify the molecular mechanism of GABA antagonists pesticides effects on the fishes, and the affinity with pesticide molecules, bioinformatic methods were used to search GABAA R β3 gene sequences that had released in the NCBI genome databases and did homologous alignment. Degenerate primers were designed in the highly conservative district. The full length cDNA sequence of GABAAR β3 of Carassius auratus was cloned successfully by RT-PCR and RACE PCR technology. The gene is 2 767 bp ( GenBank ID: KC964110 ) , with an open reading frame ( ORF) of 1 506 bp, encoding 502 amino acids. Multiple sequence alignment indicated that it was highly conserved with other known β3 subunits. QRT-PCR amplification was used to detect the activity of transcription of the gene in different tissues and organs of C. crassius. The results indicated that it was differentially expressed in organs of Carassius auratus.关键词
鲫鱼/γ-氨基丁酸/β3亚基/基因克隆/农药靶标Key words
Crassius crassius/γ-aminobutyric acid/β3 submit/cloning/pesticide target分类
农业科技引用本文复制引用
秦菠,张博,周艳芬,汪小芬,曹振龙,任天瑞,王振山..鲫鱼γ-氨基丁酸受体β3亚基基因克隆及其分析[J].农药学学报,2014,(5):508-516,9.基金项目
国家自然科学基金项目(21172147,31171191) （21172147,31171191）
上海市科委基金项目(11ZR1426800) （11ZR1426800）
河北省自然科学基金项目(C2012201106) （C2012201106）
