农药学学报Issue(2)：235-240,6.DOI:10.3969/j.issn.1008-7303.2015.02.18
四种拟除虫菊酯类杀虫剂对枸杞蚜虫的毒力及对三磷酸腺苷酶和谷胱甘肽S-转移酶活性的影响
Toxicity and the effects of four pyrethroid insceticides on the activity of ATPase and GSTs in Aphis sp.
摘要
Abstract
In order to find out pesticide that can control Aphis sp. effectively, residual film method was used to determine the toxicity of four pyrethroid insecticides, and the effects of four insecticides on ATPase and glutathione S-transferases ( GSTs) in Aphis sp. were investigated. The results indicated that Aphis sp. showed the highest sensitivity to bifenthrin, with LC50 value of 4. 34 mg/L, while the LC50 values of permethrin, beta-cypermethrin and fenpropathrin were 17. 08 mg/L, 40. 50 mg/L and 184. 84 mg/L, respectively. The activity of two kinds of ATPase in Aphis sp. could be significantly inhibited by the four insecticides. When the concentration of the four pyrethroid insecticides was 1 × 10 -4 mol/L, the inhibition to Na+-K+-ATPase was higher than that to Ca2+-Mg2+-ATPase;The order of inhibition to Na+-K+-ATPase was bifenthrin > beta-cypermethrin >permethrin >fenpropathrin;While to Ca2+-Mg2+-ATPase, the inhibition of bifenthrin was the highest, with inhibition rate of 46. 41%, and the inhibition of beta-cypermethrin was the lowest, with inhibition rate of 33. 04%. The effects of four pyrehtroids on glutathione S-transferases ( GSTs) in Aphis sp. varied. Bifenthrin could induce the GSTs activity at low concentration, but inhibited significantly at high concentration; Both beta-cypermethrin and permethrin inhibited the activity of GSTs, and the maximum inhibition rate was 85. 02%;Meanwhile, the activity of GSTs increased by 193. 07% -249. 96% after Aphis sp. was treated with fenpropathrin.关键词
枸杞蚜虫/拟除虫菊酯类杀虫剂/毒力/三磷酸腺苷酶/谷胱甘肽S-转移酶Key words
Aphis sp./pyrethroid insecticide/toxicity/ATPase/GSTs分类
农业科技引用本文复制引用
张丛,张海珠,常静,李海平..四种拟除虫菊酯类杀虫剂对枸杞蚜虫的毒力及对三磷酸腺苷酶和谷胱甘肽S-转移酶活性的影响[J].农药学学报,2015,(2):235-240,6.基金项目
国家自然科学基金(31260440) （31260440）
