热带亚热带植物学报Issue(3)：242-249,8.DOI:10.3969/j.issn.1005-3395.2014.03.005
赤桉ACT1和ACT2基因的克隆与生物信息学分析
Cloning and Bioinformatics Analysis of ACT1 and ACT2 Genes from Eucalyptus camaldulensis
摘要
Abstract
In order to reveal the biological function of Actin in growth and development of Eucalyptus camaldulensis, two fragments were cloned from the transcriptome database of E. camaldulensis seedlings at different developmental stages, named as EC-ACT1 and EC-ACT2, and their full-length cDNA were obtained by RACE method. Bioin-formatics analysis showed that the full-length cDNA of EC-ACT1 and EC-ACT2 were 1533 bp and 1387 bp, respectively, and both with 1131 bp ORF encoding 377 amino acids. The amino acid sequence of Actin in E. camaldulensis had high similarity to those of other plants with typical conserved domains of Actin. Therefore, the EC-ACT1 and EC-ACT2 genes might play a role in regulating growth and development of E. camaldulensis.关键词
赤桉/Actin基因/克隆/生物信息学分析Key words
Eucalyptus camaldulensis/Actin/Clone/Bioinformatics analysis引用本文复制引用
陈鸿鹏,朱凤云,吴志华,谢耀坚..赤桉ACT1和ACT2基因的克隆与生物信息学分析[J].热带亚热带植物学报,2014,(3):242-249,8.基金项目
广东省林业科技创新专项基金项目(2011KJCX019)；桉树生态经营及产业升级关键技术研究项目(201104003)；湛江市科技计划项目(2013A03016)；湛江市科技计划项目(2012C02139)资助 （2011KJCX019）
