热带亚热带植物学报Issue(3)：257-262,6.DOI:10.3969/j.issn.1005-3395.2014.03.007
四种添加物对铁皮石斛原球茎生长及多糖含量的影响
Effect of Four Additives on Growth and Polysaccharide Content of Dendrobium officinale Protocorm-like Bodies
摘要
Abstract
In order to understand the function of additive in medium of Dendrobium officinale, four additives, including coconut meat, sugarcane bagasse, banana peel and wheat bran, were supplied in 1/2MS medium, respectively. The effects of additives with different concentrates and culture time on growth and polysaccharide content of D. officinale PLBs were studied. The results showed that the PLBs proliferation, differentiation and the polysaccharide content were inlfuenced by four additives. The proliferation rate of PBLs reached up to 146.1%supplemented with 15.0 g L-1 sugarcane bagasse and cultured for 60 d. The polysaccharide content of PLBs was increased for 50.4%supplemented with 20.0 g L-1 sugarcane bagasse and cultured for 40 days. So it was suggested that sugarcane bagasse was suitable additive for in vitro D. officinale PLBs owing to promote the growth of D. officinale PLBs and reduce cost of production.关键词
铁皮石斛/添加物/原球茎/多糖Key words
Dendrobium officinale/Additive/PLB/Polysaccharide引用本文复制引用
包英华,潘超美,白音..四种添加物对铁皮石斛原球茎生长及多糖含量的影响[J].热带亚热带植物学报,2014,(3):257-262,6.基金项目
广东省科技计划项目(2011B060400004)资助 （2011B060400004）
