热带亚热带植物学报Issue(4):373-382,10.DOI:10.3969/j.issn.1005-3395.2014.04.008
响应面法优化超声辅助提取紫色小白菜花青苷的工艺研究
Ultrasonic Condition Optimization for Anthocyanin Extraction from Purple Cabbage by Using Response Surface Methodology (RSM)
摘要
Abstract
The aim was to optimize the ultrasonic extraction method for anthocyanins from purple cabbage [Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee] leaves. Under the conditions of 420 W ultrasonic power, 40%ethanol and twice extraction, the interactive effects of four crucial parameters, including pH, ratio of solid to liquid, ultrasonic time and ultrasonic temperature, were studied by central composite (Box-Behnken) and response surface methodology (RSM). The results showed that the optimum process for anthocyanin extraction was 1:16 of solid to liquid with pH 2.76 extracted at 58℃for 12 min under 420 W ultrasonic power. Under this condition, the extract yield of anthocyanins from purple cabbage leaves reached up to 4.11 mg g-1 DW.关键词
紫色小白菜/花青苷/超声波/提取工艺/响应面法Key words
Purple cabbage/Anthocyanins/Ultrasonic/Extract/Response surface methodology引用本文复制引用
杨碧云,叶丽萍,林琳琳,汪镇坤,邹伟明,曾晓兰,钟凤林,林义章..响应面法优化超声辅助提取紫色小白菜花青苷的工艺研究[J].热带亚热带植物学报,2014,(4):373-382,10.基金项目
福建省大宗蔬菜产业体系项目(2012K83139294);福建省自然科学基金项目(2012J01082);福建农林大学校重点建设项目(6112c0409)资助 (2012K83139294)