热带亚热带植物学报Issue(1)：37-42,6.DOI:10.11926/j.issn.1005-3395.2015.01.006
细枝木麻黄离体培养过程中愈伤组织和再生芽的细胞组织学观察
Cytohistological Observation of Callus and Adventitious Buds of Casuarina cunninghamianaMiq.in vitro
摘要
Abstract
In order to understand the cytohistology ofCasuarina cunninghamianacallus, cell division, callus differentiation and adventitious bud regeneration were studied under scanning electron microscope and parafifn sections. The results showed that parenchyma cells in the wound started dedifferentiation in the callus induction medium. After cultured for one week, callus were induced at the explant wound, and embryonic callus was formed. Bud primordium was induced from surface cells after two weeks. After culturing for a further four weeks, embryonic bud primordium became more increasely visible, and gradually differentiated to become adventitious bud. After another six weeks, most of the adventitious buds have proliferated and differentiated, and some developed to shoots. It was conifrmed that the regeneration ofC. cunninghamianacould be achieved through embryoid callus differentiation, it would provide basis for establishment of efifcient regeneration system inC. cunninghamina.关键词
细枝木麻黄/离体培养/愈伤组织Key words
Casuarina cunninghamianaMiq./in vitroculture/Callus引用本文复制引用
姜清彬,仲崇禄,陈羽,刘芬,张勇,陈珍..细枝木麻黄离体培养过程中愈伤组织和再生芽的细胞组织学观察[J].热带亚热带植物学报,2015,(1):37-42,6.基金项目
中央公益性科研院所基金项目(RITFYWZX201203)；“十二五”林业科技支撑计划项目(2012BAD01B0603)资助 （RITFYWZX201203）
