热带亚热带植物学报Issue(3)：227-235,9.DOI:10.11926/j.issn.1005-3395.2015.03.001
落地生根S-腺苷高半胱氨酸水解酶基因的克隆及生物信息学分析
Cloning and Bioinformatics Analysis of S-adenosylhomocysteine Hydrolase Gene formKalanchoe daigremontiana
摘要
Abstract
In order to understand the function ofSAHHinKalanchoe daigremontiana, a cDNA sequence, named asKdSAHH, was cloned by RT-PCR and RACE-PCR. The results showed that the full-length ofKdSAHHcDNA was 1748 bp, encoding 485 amino acids. The predicted molecular weight (MW) of KdSAHH was about 53 kDa with estimated pI of 5.59–5.682. The Scanprostie and DNAstar prediction showed that KdSAHH protein with two conserved motifs was very conservative in evolution. There were high homology between KdSAHH and SAHHs in other species by amino acid alignment, and which had the closest relationship with that inMedicago sativa. Lupinus luteusused as template, there were differences in three-dimensional structure of KdSAHH simulated by SWISS-MODLE and Phyre. These would lay out basis for research in expression and function of KdSAHH.关键词
落地生根/S-腺苷高半胱氨酸水解酶/SAHH/克隆/生物信息学Key words
Kalanchoe daigremontiana/S-adenosylhomocysteine hydrolase/SAHH/Clone/Bioinformatics引用本文复制引用
苏振声,苏文锋,杨秉建,张利娟,李亚超,白宇清,闫纯,曾会明,钟天秀..落地生根S-腺苷高半胱氨酸水解酶基因的克隆及生物信息学分析[J].热带亚热带植物学报,2015,(3):227-235,9.基金项目
深圳市科技计划项目(CXZZ20140418110342522)资助 （CXZZ20140418110342522）
