热带亚热带植物学报Issue(3)：245-251,7.DOI:10.11926/j.issn.1005-3395.2015.03.003
麻竹miR172a靶基因DlAP2的克隆及其表达
Cloning and Expression Analysis ofmiR172a Targeted GeneDlAP2in Dendrocalamus latiflorus
摘要
Abstract
In order to understand the function ofDlAP2inDendrocalamus latiflorus, onemiR172a targeted gene named asDlAP2was cloned fromD. latiflorusby RT-PCR and RACE. Sequence analysis showed that the full length cDNA ofDlAP2was 1729 bp, including 5′ untranslated region (UTR) 81 bp, open reading frame (ORF) 1464 bp, 3′ UTR 351 bp, 24 bp polyA, and onemiR172a complementary site (CTGCAGCATCATCAGGATTCT) at 130 bp of the 3′ end in ORF.DlAP2encodes a putative protein with 487 amino acids with two AP2 domains, which indicate that it belongs to AP2 group of AP2 subfamily in AP2/ERF family. DlAP2 has a high homology with those AP2 from other monocots. RLM-5′ RACE analysis showed thatDlAP2was regulated bymiR172a through cutting mainly at the site between the 11th and 12th bases. Real-time quantitative PCR results showed that the expression pattern ofDlAP2was opposite to that ofmiR172a in lfower buds. These validated thatmiR172a played a regulatory role in regulating the expression ofDlAP2.关键词
麻竹/AP2基因/miR172a/基因表达Key words
Dendrocalamus latiflorus/AP2gene/miR172a/Gene expression引用本文复制引用
高志民,娄永峰,王丽丽,杨丽,赵韩生,陈东亮..麻竹miR172a靶基因DlAP2的克隆及其表达[J].热带亚热带植物学报,2015,(3):245-251,7.基金项目
国家林业局948项目(2011-4-55)资助 （2011-4-55）
