四川大学学报（自然科学版）Issue(1)：163-169,7.DOI:10.3969/j.issn.0490-6756.2015.01.029
多重PCR检测病死鸡中沙门氏菌方法的研究
Study on the detection of Salmonella from dead chicken by multiplex PCR
摘要
Abstract
To establish the multiplex PCR which could detect Salmonella,Salmonella pullorum and Salmonella enteritidis,simultaneously.The genomic DNA of bacteria was extracted by the boiling method,and the specific genes (invA,fliC,sdfⅠ)of Salmonella,Salmonella pullorum and Salmo-nella enteritidis were selected to disign primers for establishing the multiplex PCR method ,and then the specificity and sensitivity of multiplex PCR were tested;At the same time,3 6 9 samples from 1 7 farms were detected by multiplex PCR,the results of the test were compared with the traditionaldetec-tion methods.The results showed that the Salmonella species could be tested simultaneously by the op-timized multiplex PCR,its sensitivity d was 4.6×102 CFU/mL;The number of Salmonella,Salmonel-lapullorum and Salmonellaenteritidis which detected by the multiplex PCR was 34,21 and 5 ,respec-tively,the coincidence rate with traditional bacteria culture method was 91.2%,90.5% and 80.0% , respectively.关键词
多重PCR/沙门氏菌/鸡白痢沙门氏菌/肠炎沙门氏菌Key words
Multiplex PCR/Salmonella/Salmonella pullorum/Salmonella enteritidis分类
农业科技引用本文复制引用
杨帆,王红宁,张安云,雷昌伟,张志坤..多重PCR检测病死鸡中沙门氏菌方法的研究[J].四川大学学报（自然科学版）,2015,(1):163-169,7.基金项目
国家基础研究计划973项目(2013CB127200) （2013CB127200）
国家公益性行业(农业)科研专项经费项目(201303044),(201403054) （农业）
国家“现代农业产业技术体系建设专项资金”项目(CARS-41-K09) （CARS-41-K09）
四川省公益性科研专项(2013NZ0025) （2013NZ0025）
四川省蛋鸡产业链项目(2011NZ0073) （2011NZ0073）
四川省肉鸡产业链项目(2012NZ0037) （2012NZ0037）
