四川大学学报（自然科学版）Issue(1)：181-186,6.DOI:10.3969/j.issn.0490-6756.2015.01.032
一种快速检测蛋鸡沙门氏菌的方法
A rapid method for detection of Salmonella in laying hens
摘要
Abstract
The aim of this study was to develop a method for the rapid and specific detection of Salmonel-la in the fecal swabs of laying hens.The composition of buffered peptone water (BPW)was modified in order to develop a one-step 6-hr-long culturing selective enrichment medium (SEM),and a specific prim-er hilAP3 was designed with consideration of the interference of a variety of non-Salmonella bacteria in hen’s feces.The results showed that,after cultivation for 6 h,the growth of low quantity (1-5 CFU/50 mL)Salmonella in SEM reached 103 CFU/mL,which were well above the typical detection limit of PCR (1 × 102 CFU/mL).Primer hilAP3 was proved to be specific for PCR amplification of Salmonella from feces of laying hens.By using this 9-hr-long method that involves one step selective culturing (6 h)and a specific primer PCR assay,as few as 1-5 CFU Salmonella per 50 mL enrichment broth could be detec-ted,and its accuracy was 100%.Hence,this optimized 9 h method is rapid,sensitive and specific,and may serve as an effective method for detection of Salmonella in fecal samples of laying hens at a large scale.关键词
蛋鸡/沙门氏菌/增菌液优化/快速检测Key words
Laying hens/Salmonella/Enrichment medium optimization/Rapid detection分类
农业科技引用本文复制引用
李婉丽,徐文龙,李松,余洋,孙群..一种快速检测蛋鸡沙门氏菌的方法[J].四川大学学报（自然科学版）,2015,(1):181-186,6.基金项目
四川省科技支撑计划(2014NZ0106) （2014NZ0106）
肉鸡现代产业链关键技术集成研究与产业化(2012NZ0037) （2012NZ0037）
国家自然科学基金(J1103518) （J1103518）
