四川精神卫生Issue(1)：17-21,5.DOI:10.11886/j.issn.1007-3256.2015.01.006
成年大鼠局灶脑缺血/再灌注后海马BDNFmRNA和bFGF mRNA的动态变化研究
Study on dynamic changes of BDNF mRNA and bFGF mRNA in hippocampus of adult rats with focal cerebral ischemia/reperfusion
摘要
Abstract
Objective To study on dynamic changes of BDNF mRNA and bFGF mRNA in hippocampus of adult rats with focal cere-bral ischemia/reperfusion. Methods The animal model of focal cerebral ischemia/reperfusion was made by filament occlusion of the right middle cerebral artery. 108 male Wistar rats were randomly divided into three groups:normal control group(NC group),sham operation con-trol group(SC group),ischemia/reperfusion group(I/R group),each group contain 1,3,7,14,21 and 28d six time points(for each point,n=6). In situ hybrization method was used to detect the number of BDNF mRNA and bFGF mRNA expression positive cells following various time in hippocampus of adult rats. Results Normally a small amount of BDNF mRNA and bFGF mRNA in hippocampus. After focal cerebral ischemia/reperfusion,the number of BDNF mRNA and bFGF mRNA positive cells increased at each time point,reached small peak value after 3 days(P<0. 01). Immunoprecipitates were dyed deeply,after 7 days decreased rapidly,28d decreased to normal level(P<0. 05). Conclusion Focal cerebral ischemia/reperfusion can up-regulated expression of BDNF mRNA and bFGF mRNA,Advantageous to the recovery of neural function after cerebral ischemia and have neuroprotective effect.关键词
局灶脑缺血/再灌注/BDNFmRNA/bFGF mRNA/海马/大鼠Key words
Focal cerebral ischemia/reperfusion/BDNF mRNA/bFGF mRNA/hippocampus/Rat分类
医药卫生引用本文复制引用
黄艳君,罗勇,张中念,曹飞虎,董明,宋晓灵,张弟文..成年大鼠局灶脑缺血/再灌注后海马BDNFmRNA和bFGF mRNA的动态变化研究[J].四川精神卫生,2015,(1):17-21,5.基金项目
四川省卫生厅科研课题（080211）。 （）
