四川精神卫生Issue(2)：116-118,119,4.DOI:10.11886/j.issn.1007-3256.2015.02.007
氯丙嗪致大鼠便秘与炎性细胞因子表达的对照研究
Expression of cytokines is partially involved in the constipation induced by chlorpromazine
张家瑞 1任丽娜 1赵均铭 2张勇1
作者信息
- 1. 300222 天津市精神卫生中心
- 2. 中国医学科学院血液病医院血液学研究所
- 折叠
摘要
Abstract
Objective To investigate the effects of chlorpromazine on bowel movements as well as intestinal inflammation in rats and uncover the related mechanisms. Methods Fifty male SD rats were randomly divided into 5 groups including normal control,low middle and high dose of chlorpromazine,as well as neostigmine treated chlorpromazine group. After two weeks of the application of chlorpromazine,the effects of Chlorpromazine on rat bowel movements and intestinal propulsion were studied;the expressions of induc-ible nitric oxide(iNOS)synthase,cyclooxygenase - 2(COX - 2),NF - κB were respectively studied using ELISA and Western blot-ting methods. Results After two weeks of chlorpromazine application(40 mg/ kg),chlorpromazine significantly decreased the fecal frequency and water from(81 ± 15)% ,(60 ± 13)% to(33 ± 21)% ,(25 ± 27)%(P < 0. 05);the corresponding colonic cytokines and inflammatory mediators were significantly increased,which is associated with increased protein expressions of iNOS,COX - 2,NF- κB compared with normal control rats from(100 ± 11)% ,(100 ± 9)% ,(100 ± 12)% to(289 ± 19)% ,(311 ± 28)% ,(209 ± 18)%(P < 0. 05). Conclusion Chronic use of chlorpromazine leads to colon inflammation through NF - κB activating iNOS/ COX -2 pathway. Chlorpromazine is also widely used in Long - term hospitalization and economic difficulties of the patients.关键词
氯丙嗪/炎症/COX - 2/iNOSKey words
Chlorpromazine/Inflammation/iNOS/COX - 2分类
医药卫生引用本文复制引用
张家瑞,任丽娜,赵均铭,张勇..氯丙嗪致大鼠便秘与炎性细胞因子表达的对照研究[J].四川精神卫生,2015,(2):116-118,119,4.
