四川医学Issue(5)：545-547,548,4.
类固醇受体辅活化子-3在烧伤后T淋巴细胞功能抑制中的作用
Study on the Role of Steroid Receptor Coactivator-3 in the Dysfunction of T Lymphocytes after Burn Injury in Mice
摘要
Abstract
Objective To investigate the effect of steroid receptor coactivator ( SRC)-3 on T lymphocyte function after severe burns.Methods 10 female healthy SPF-grade SRC-3 +/ +mice and SRC-3-/-mice respectively, 20g and are named S group and S-group.Each group was divided into two phase points:normal (N) and 24h, which each of points had 5 mice.The distribution of T lymphocyte subsets of peripheral blood, spleen, thymus, and the levels of IL-2 and soluble IL-2 receptor ( sIL-2R) were deter-mined.Results There were no statistical significance between two groups about the levels of plasma IL-2 and sIL-2R under normal condition.They were both increased significantly (P<0.01) at 24h after burn in two groups, but the plasma IL-2 level was signifi-cantly lower in the S-group than that of S group (P<0.01) and the plasma sIL-2R content was markedly higher in the S-group than that of S group.Under normal condition, CD3 +, CD4 + and the ratio of CD4 + /CD8 + of peripheral blood, spleen and thymus were lower in the S-group than those of S group (P<0.05 or P<0.01), but spleen CD8 + was higher in the SRC-3-/-group than that of S group (P<0.01).After burn, the CD3 +, CD4 + and CD4 + /CD8 + ratio of peripheral blood, spleen and thymus were both de-creased (P<0.05 or P<0.01), the CD8 + were significantly increased (P<0.05 or P<0.01).Compared with the S group, the CD3 + and CD4 + of peripheral blood, spleen and thymus were decreased significantly ( P<0.01 ) , and there were no significant differences in the ratio of CD4 + /CD8 + in the S-group.Conclusion The SRC-3 protein might be related to the inhibition of T lym-phocyte immune function after severe burns, which its deletion can cause the distribution changes of T lymphocyte subsets.关键词
类固醇受体辅活化子-3/淋巴细胞亚群/IL-2/可溶性白介素-2受体Key words
steroid receptor coactivator-3/T-lymphocyte subsets/IL-2/sIL-2R分类
医药卫生引用本文复制引用
李军,林露,汪海洋,冉茂荣,刘英海,安虹,牛洁,王军平,粟永萍..类固醇受体辅活化子-3在烧伤后T淋巴细胞功能抑制中的作用[J].四川医学,2014,(5):545-547,548,4.基金项目
成都军区总医院院管课题(2011YG-B09)和创伤、烧伤与复合伤国家重点实验室开放基金(SKLKF200910) （2011YG-B09）
