四川医学Issue(7)：779-781,3.
胃转流术对2型糖尿病大鼠内脏脂肪组织 vaspin mRNA 表达的影响
Effecfs of Gastric Bypass on Expression of Vaspin mRNA in Omental Adipose Tissue of Type 2 Diabetes Mellitus Rats
摘要
Abstract
Objective To investigate the effects of gastric bypass( GBP) on expression of vaspin mRNA in omental adi-pose tissue of type 2 diabetes mellitus(T2DM) rats. Methods The models of T2DM rats were induced by Stre- ptozotocin and high-fat, randomly divided into 2 groups: T2DM-GBP group( A group), T2DM-sham operation( B group). 20 age-and sex-matched normal SD rats were randomly divided into 2 groups: GBP group(C group),and Sham operation(D group). Fasting plas-ma glucose (FPG)level was determined before operation and on week 4,8 after operation,respectively. At the same time,Rats o-mental adipose tissue was harvested eight weeks postoperatively and analyzed by using reverse transcription polymerase chain reac-tion (RT-PCR) to detect the vaspin mRNA expression. Results The FPG level progressively decreased in the A group,which was significant differences of the indicator between before operation and on week 4,8 after operation((P < 0. 05). Compared the indicator after operation with before operation,the FPG level was not statistically significant( P > 0. 05) in the B group,C group,or D group. Eight weeks after surgery, vaspin mRNA expression was no significant difference between A group, C group and D group. (P > 0. 05), which of the B group rats adipose tissue was higher than those in A group, C group,or D group. Conclusion GBP surgery can significantly decrease the fasting plasma glucose of T2DM rats, Maybe the low expression of vaspin mRNA in omental adipose tissue after GBP surgery play an important role in decreasing the FPG of T2DM rats.关键词
2型糖尿病/胃转流术/血糖/Vaspin mRNAKey words
type 2 diabetes mellitus/gastric bypass surgery/plasma glucose/vaspin mRNA分类
医药卫生引用本文复制引用
唐锦,何劲松,王崇树,何一,魏寿江..胃转流术对2型糖尿病大鼠内脏脂肪组织 vaspin mRNA 表达的影响[J].四川医学,2014,(7):779-781,3.基金项目
川北医学院校级课题(编号:CBY12-A-QN16) （编号:CBY12-A-QN16）
