四川医学Issue(7)：782-784,3.
碱性成纤维细胞生长因子缓释微球对许旺细胞在小肠粘膜下层增殖活性的影响
Study on the Activities of SC Proliferation with Basic Fibroblast Growth Factor Controlled Release Microspheres Combined with Schwann Cells and Small Intestinal Submucosa
摘要
Abstract
Objective To observe the effects of basic fibroblast growth factor( bFGF) micropheres on adhesion and pro-liferation and migration of schwann cells(SC) on scaffold of small intestinal submucosa(SIS), and the activities of SC proliferation was observed. Methods Schwann cells was isolated ,cultured and purified in vitro using two-step enzymatic digestion method, To explore the effects of bFGF- PLGA- MS on adhesion and proliferation and migration of schwann cell(SC) on the scaffolds of SIS in vitro, and the free bFGF group and simple medium group was used as control. Results A larger number of active Schwann cells with the cell purity above 90% were obtained using two-step enzyme digestion method a of primary cells culture in vitro, the good activity of cells in vitro were confirmed, the cell cycle is about 6 ~ 7 days and the logarithmic growth phase is 2 ~ 7 days after cu-lutre, The bFGF-MS was observed continuously promoting the adhesion and proliferation and migration of Schwann cells on SIS, the cell cycle of SC be shortened by bFGF-MS with a more stable and good activity of cells, according to flow cytometry, the bFGF microspheres increased cell proliferation index, and continuously maintained a higher proliferative activity of SC on SIS, it showed a clear positive correlation between the content of bFGF released from microspheres and cells counts, with the correlation coeffi-cient of 0. 988 (P = 0. 02). Conclusion The drug delivery of bFGF-PLGA-MS promotes Schwann cells sustained and stable ad-hesion and proliferation on the scaffold of small intestinal submucosa, matched with the drug release rate. Schwann cells are provid-ed to be constructed artificial nerve combined with SIS.关键词
碱性成纤维细胞生长因子/缓释微球/bFGF-PLGA 缓释微球/许旺细胞/小肠粘膜下层/增殖Key words
basic fibroblast growth factor/controlled release microsphere/bFGF-PLGA-MS/schwann cell/small intesti-nal submucosa/proliferation分类
医药卫生引用本文复制引用
肖睿,张开伟,李同相,项舟,段宏,舒运兵..碱性成纤维细胞生长因子缓释微球对许旺细胞在小肠粘膜下层增殖活性的影响[J].四川医学,2014,(7):782-784,3.基金项目
宜宾市重点科技计划项目(项目编号:200803010) （项目编号:200803010）
