四川医学Issue(3)：309-312,4.DOI:10.16252/j.cnki.issn1004-0501-2015.03.012
PI-103对人黑色素瘤A375细胞增殖和侵袭的影响
Effects of PI-103 on Proliferation and Invasion of Human Melanoma A375 Cells
摘要
Abstract
Objective To investigate the effects of PI-103 on the proliferation and invasion of human melanoma A375 cells in vitro and its mechanism. Methods The effects of PI-103 with different concentration on the proliferation of A375 cells were detected by MTT assay. The morphological changes of A375 cells were observed by phase contrast microscope. The effects of PI-103 at 0. 1, 0. 2μmol/L concentration on the invasion of A375 cells were detected by transwell chamber invasion experiment. The expressions of MMP-2 and MMP-9 of A375 cells were determined by Western Blot. Results The proliferation of A375 cells treated with PI-103 at the concentration higher than 0. 4μmol/L was significantly inhibited(P<0. 05)and the typical morphologi-cal changes of apoptosis were seen under phase contrast microscope. The numbers of transmembrane cells in the invasion experi-ment treated with PI-103 at 0. 1, 0. 2μmol/L concentration were significantly decreased(P<0. 05)and the expressions of MMP-2 and MMP-9 were significantly declined(P<0. 05). Conclusion PI-103 can inhibit the proliferation and invasion of A375 cells, which is expected to be a potential anti-tumor drug in treatment of malignant melanoma.关键词
PI-103/黑色素瘤/增殖/侵袭Key words
PI-103/melanoma/proliferation/invasion分类
医药卫生引用本文复制引用
陆茂,叶俊儒,彭科,宋黎..PI-103对人黑色素瘤A375细胞增殖和侵袭的影响[J].四川医学,2015,(3):309-312,4.基金项目
四川省卫生厅科研课题(编号:110466) （编号:110466）
