四川医学Issue(3)：316-319,4.DOI:10.16252/j.cnki.issn1004-0501-2015.03.014
四川省德阳地区散发性戊型肝炎临床特点及病毒基因型
Distribution and Clinical Characteristics of Hepatitis E Virus Genotypes in Sichuan Deyang District
摘要
Abstract
Objective To investigate the distribution and clinical features of Hepatitis E Virus genotypes in Deyang dis-trict,Sichuan Province. Methods Serum samples from 154 acute HEV patients were tested by biochemical methods and ELISA. Total RNA were extracted from serum samples and used for PCR amplification. PCR positive products were bi-directional se-quenced. Clustal X and MEGA 4. 1 were used for nucleotide alignment and phylogenetic analysis. Results Among these 154 a-cute HEV patients,111(72. 1%)were male and 43(27. 9%)were female. The average age of these patients was 45. 6 ± 16. 3 and 77. 9% of these patients aged from 20 to 59 years old. Among 92 hospitalized patients,49,42 and 1 was infected with HEV,HEV plus HBV and HEV plus HCV,respectively. Two patients infected with HEV developed into acute liver failure and both of them were dead. In patients co-infected with HEV and HBV,11 patients developed into acute liver failure and 10 of them were dead. The differences of occurrence rates of liver failure and fatality rates between patients infected with HEV and HEV plus HBV were <br> statistically significant(P<0. 05). HEV RNA was detected in 34 patients and the positive rate was 21. 4%. Nucleotide alignment and phylogenetic analysis of HEV strains from these 34 patients and standard viral strains with the use of Clustal X and MEGA showed that similarities of these 34 HEV strains were 76. 6% ~98% and their homology rates with genotype IV standard strains were higher than with other standard strains. Taken together,our results revealed that HEV strains detected in our district belonged to genotype IV. Conclusion Genotype IV was the dominant genotype in Deyang district,Sichuan province. Compared with patients infected with HEV,patients co-infected with HEV and HBV were more likely to develop into severe liver diseases.关键词
戊型肝炎/病毒基因型/分布/HEV RNAKey words
hepatitis E/genotype/distribution/HEV RNA分类
医药卫生引用本文复制引用
杨家红,张浩,陈学兵,陈皋,王秀..四川省德阳地区散发性戊型肝炎临床特点及病毒基因型[J].四川医学,2015,(3):316-319,4.基金项目
四川省卫生厅立项科研课题(编号:060228) （编号:060228）
