四川医学Issue(4)：470-473,4.DOI:10.16252/j.cnki.issn1004-0501-2015.04.010
富血小板血浆体外抑菌作用研究
The Investigation of Antibacterial Effects of Platelet-rich Plasma in Vitro
摘要
Abstract
Objective To study the antibacterial effects of platelet rich plasma in vitro. Methods Prepare platelet-rich plasma( PRP) , platelet-poor plasma( PPP) , Use the liquid LB medium as negative control,observe the antibacterial effect of PRP and PPP under the condition of 37 ℃ and 22 ℃ respectively at different times (2h, 4h, 6h, 8h, 12h, 24h)against Staphylococ-cus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa. Results Under the condition of 37 ℃ and 22 ℃,both PRP and PPP showed good antibacterial effects on the growth of Staphylococcus aureus and Staphylococcus epidermidis, which was time-bound. For Staphylococcus aureus, the maximum bacteriostatic effect appeared during 2 ~ 4h;the bacteriostatic effect of PPP decreased after 4h, while PRP maintained the good bacteriostatic effect which could sustainable to 12h;neither PRP or PPP had antibacterial effect 24h later. The bacteriostatic effect of PRP and PPP against Staphylococcus epidermidis could sustainable to 24h. There was no significant relationship between the concentration of platelet and antimicrobial effect. Either PPP or PRP showed no antibacterial activity against Pseudomonas aeruginosa. Conclusion Platelet rich plasma has specific antibacterial spectrum, and the bacteriostatic effect of different bacterial species is time-bound.关键词
富血小板血浆/抑菌作用Key words
platelet rich plasma/bacteriostasis分类
医药卫生引用本文复制引用
肖洁,李翠莹,张利,杨明,何哲文,唐皎雪..富血小板血浆体外抑菌作用研究[J].四川医学,2015,(4):470-473,4.基金项目
四川省卫生厅科研课题(编号:110474) （编号:110474）
