四川医学Issue(4)：484-487,4.DOI:10.16252/j.cnki.issn1004-0501-2015.04.014
SCCmec相关psm-mec在临床分离表皮葡萄球菌中的分布和特征分析
Distribution and Characteristics of SCCmec Associated-psm-mec in Staphylococcus Epidermidis of Clinical Isolates
杨永长 1陈亮 1肖代雯 1喻华 1刘华 1黄文芳1
作者信息
- 1. 四川省医学科学院·四川省人民医院检验科，四川 成都610072
- 折叠
摘要
Abstract
Objective To investigate the distribution and characteristics of SCCmec-associated psm-mec in Staphylococ-cus epidermidis of clinical isolates,and to lay a foundation for further study on its function of psm-mec in Staphylococcus epidermi-dis. Methods 84 strains of Staphylococcus epidermidis were collected and identified by full automation microbiological identifica-tion system. Esp and mecA genes were amplified by PCR to differentiate methicillin-resistant Staphylococcus epidermidis ( MRSE) . Psm-mec and fudoh genes were used to determine psm-mec positive strains,and the distribution of psm-mec in MRSE i-solated from different sample type was analyzed. The regions from mecR1 to psm-mec and from psm-mec to xylR were amplified to explore the relationship between psm-mec and mecR1,xylR. Results PCR results of esp and mecA showed that there were 64 strains of MRSE with a rate of 76. 19%. 25 psm-mec positive strains mainly from blood and sputum were confirmed by the amplifi-cation results of psm-mec and fudoh genes, and the rate of psm-mec in MRSE was 39. 0%( 25/64 ) . And no psm-mec positive strains were found in methicillin-sensitive Staphylococcus epidermidis. In 25 strains of MRSE with psm-mec,all strains were posi-tive to mecR1/psm-mec,and only 16 strains positive to psm-mec/xylR. Conclusion SCCmec-associated psm-mec exists in MRSE of clinical isolates. Psm-mec connects with mecR1 in all strains,but connects with xylR only in 64% strains.关键词
聚合酶链反应/psm-mec基因/耐甲氧西林表皮葡萄球菌Key words
polymerase chain reaction/psm-mec gene/methicillin resistant staphylococcus epidermidis分类
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杨永长,陈亮,肖代雯,喻华,刘华,黄文芳..SCCmec相关psm-mec在临床分离表皮葡萄球菌中的分布和特征分析[J].四川医学,2015,(4):484-487,4.
