四川医学Issue(6)：822-824,825,4.DOI:10.16252/j.cnki.issn1004-0501-2015.06.017
CD28/CTLA-4共刺激分子在实验性变态反应性脑脊髓炎发病机制中的作用
Role of CD28/CTLA-4Costimulatory Molecules in the Pathogenesis of Experimental Allergic Encephalomyelitis
吕志宇 1李晓红 1陈秀 1李作孝1
作者信息
- 1. 泸州医学院附属医院神经内科，四川 泸州646000
- 折叠
摘要
Abstract
Objective To study the CD28/CTLA-4 costimulatory molecules in the pathogenesis of experimental allergic encephalomyelitis( EAE) . Methods 60 adult female guinea pigs were randomly divided into 3 groups with 20 rats in each group, the normal control group,adjuvant control group and EAE model group were named. The percentage of CD28,CTLA-4 expressing on CD 4+T cells were detected by FACS and INF-γ,IL-4 secreted by PBMC were detected by ELISA. Results The percentage of CD28 in EAE model group was(42. 39 ± 9. 82)%,higher than that of normal control group and adjuvant control group,the differ-ence was statistically significant(P<0. 05);the percentage of CTLA-4 in EAE model group was(2. 31 ± 1. 27)%,lower than that of normal control group and adjuvant control group,the difference was statistically significant(P<0. 05);the normal control group and adjuvant control group in both the expression of CD28 and CTLA-4 rate were not statistically different(P>0. 05). Analysis of Pearson linear correlation discovery:CD28/CTLA-4 ratio and IFN-γ/IL-4 ratio was positively related(r=0. 645,P=0. 000). Con-clusion The imbalance of CD28/CTLA-4 costimulatory molecules and the downstream imbalance of Th1/Th2 were occurred in rats with EAE,Which results occurrence of the disease.关键词
实验性变态反应性脑脊髓炎/共刺激分子/CD28/CTLA-4/T辅助淋巴细胞Key words
experimental allergic encephalomyelitis/costimulatory molecules/CD28/CTLA-4/T helper lymphocytes分类
医药卫生引用本文复制引用
吕志宇,李晓红,陈秀,李作孝..CD28/CTLA-4共刺激分子在实验性变态反应性脑脊髓炎发病机制中的作用[J].四川医学,2015,(6):822-824,825,4.
