中国伤残医学Issue(14)：11-13,3.DOI:10.13214/j.cnki.cjotadm.2014.14.007
大鼠Ngn2基因真核表达质粒的构建
Construction of rat Ngn2 eukaryotic expression plasmid
摘要
Abstract
Objective:To construct eukaryotic expression plasmid of rat Neurogenin 2 (Ngn2).Methods:The total RNA was extracted from the hippocampus of rats .The cDNA encoding Ngn 2 gene was amplified by RT-PCR, cloned and constructed the recombinant plas-mid pSecTag2/HygroB-Ngn2.The recombinant plasmid was identified by restriction enzyme Xho Ⅰand Hind III cutting and sequence a-nalysis .Results:The product of RT-PCR was coincide with what we preconceived .Restriction enzyme XhoⅠand Hind III cutting and se-quence analysis proved the recombinant plasmid to be pSecTag 2/HygroB-Ngn2.It was constructed successfully .Conclusion:The success-ful cloning of rat Ngn2 gene might lay a basis for studying the effects on its nerve repair.关键词
Neurogenin2基因/真核表达质粒Key words
Neurogenin2 gene/Eukaryotic expression plasmid分类
医药卫生引用本文复制引用
白登彦,张海军,袁治国..大鼠Ngn2基因真核表达质粒的构建[J].中国伤残医学,2014,(14):11-13,3.基金项目
甘肃省科技厅科技支撑计划项目（编号1104FKCA117）；甘肃省卫生行业科研计划管理项目 （）
