中国伤残医学Issue(14)：31-33,3.DOI:10.13214/j.cnki.cjotadm.2015.14.019
CCK－8法检测牛膝醇提物诱导兔骨髓间充质干细胞最佳检测条件的实验研究
Experimental study on CCK-8 method for detection of rabbit bone marrow mesenchymal stem cells induced by Achyranthes bidentata alcohol extract for optimal detection conditions
摘要
Abstract
Objective:To study on CCK-8 method for detection of Achyranthes bidentata alcohol induced by extracts of rabbit bone marrow mesenchymal stem cells ( BMSCs) optimal detection conditions.Methods: With rabbit BMSCs isolation, culture and amplifica-tion, the amplification of the subculture, and take the fourth generation cells used in the experiments.Comparison of CCK-8 absorption spectrophotometry method of difference value in different incubation time and different wavelength detection conditions, detection of Achy-ranthes bidentata alcohol research method of CCK-8 induced by extracts of rabbit BMSCs optimal testing time and the best detection wavelength.Results:Achyranthes bidentata alcohol extract could obviously induce rabbit bone marrow mesenchymal stem cells prolifera-tion induced by BMSCs, the feasibility of rabbit cartilage differentiation had strong.CCK-8 method for detection of Achyranthes bidentata alcohol induced by extracts of rabbit BMSCs optimal testing time to join the CCK-8 reagent after incubation of the 2h, the best detection wavelength of 450nm.Suitable for detection of cell number range of3 ×103/ml~4 ×10^4/ml.Conclusion:CCK-8 method in after 2h incubation and enzyme mark instrument 450nm wavelength detection of Achyranthes bidentata alcohol extract induced rabbit BMSCs high-est sensitivity.关键词
CCK-8法/牛膝醇提物/诱导/兔BMSCs/最佳检测条件/实验研究Key words
CCK-8 method/Alcohol extractive of Achyranthis bidentatae/induced rabbit BMSCs/the best testing condition/Ex-perimental observation分类
医药卫生引用本文复制引用
陈浩雄,彭力平,徐宁达,裴军宇,刘兴利,赵亮..CCK－8法检测牛膝醇提物诱导兔骨髓间充质干细胞最佳检测条件的实验研究[J].中国伤残医学,2015,(14):31-33,3.基金项目
深圳市福田区卫生公益性科研项目 （）
