肿瘤预防与治疗Issue(3)：126-129,4.DOI:10.3969/j.issn.1674-0904.2014.03.003
水杨酸对人肝癌HepG2细胞体外生长的影响的研究*
Influence of Salicylic Acid on Human Hepatic Cancer HepG2 Cells Growth In-vitro
摘要
Abstract
Objective: Salicylic acid ( SA) and its derivatives have been shown to induce apoptosis in a variety of cancer cells. The aim of this study is to investigate influence of SA on human hepatic cancer HepG2 cells growth in-vitro. Methods:MTT assay was used to determine the effect of SA on viability of HepG2 cells, EdU assay was used to detect the impact of SA on the proliferation activity of HepG2 cells, and the cell cycle progress altered and apoptosis of HepG2 cells induced by SA were determined using flow cytometry ( FCM) . Results:SA reduced significantly viability of hepatic cancer HepG2 cells in a concentration-dependent manner and had an IC50 value of (8. 92 ± 0. 45)mmol/L;EdU assay showed that the red fluorescence produced by incorporation of EdU decreased in HepG2 cells treated with SA for 24hr, thereby depress-ing the proliferation activity of HepG2 cells;FCM assay showed that compared to control, SA induced obviously cell cycle G0/G1-phase arrest ( 65. 5% ± 1. 21% vs. 34. 3% ± 0. 89%, P <0. 05 ) and delayed in entering S phase 24. 2% ± 0. 89% vs. 44. 0% ± 0. 64%, P<0. 05), and promoted apoptosis in HepG2 cells(24. 9% ± 0. 32% vs. 2. 3% ± 0. 11%, P<0. 05). Conclusion:SA would inhibits the growth of HepG2 cells by altering cell cycle progress, depressing prolifera-tion activity and promoting cell apoptosis.关键词
水杨酸/人肝癌HepG2细胞株/细胞周期/凋亡Key words
Salicylic Acid/Human Hepatoma HepG2 Cell Line/Cell Cycle/Apoptosis分类
医药卫生引用本文复制引用
林冰,郎锦义,雷晴..水杨酸对人肝癌HepG2细胞体外生长的影响的研究*[J].肿瘤预防与治疗,2014,(3):126-129,4.基金项目
川干研2012-504 （）
