肿瘤预防与治疗Issue(4)：165-170,6.DOI:10.3969/j.issn.1674-0904.2014.04.002
氟尿嘧啶和奥沙利铂在胃癌中的体外化疗敏感性检测
In Vitro Chemosensitivity of Fluorouracil and Oxaliplatin in Gastric Cancer
摘要
Abstract
Objective: To investigate the in vitro chemosensitivity of fluorouracil (5-FU) and oxaliplatin (OX) in gastric cancer. Methods:Patients with histologically confirmed gastric cancer were enrolled for the study. All patients a-greed to the chemosensitivity test of their resected tumors and gave informed consent. Adenosine triphosphate tumor chemo-sensitivity assay ( ATP-TCA) was performed to determine the chemosensitivity of 5-FU and OX in resected gastric cancer specimens. Results:Twenty-seven patients with a median age of 60. 0 were enrolled, including 21 males and 6 females. The tumors were located in the lower part of the stomach in 14 cases, 20 cases were poorly differentiated or signet-ring cell carcinoma and 21 cases were in late TNM stage. ATP-TCA assay showed that the tumor growth inhibition rates of 5-FU+OX were significantly higher than those of 5-FU or OX at each test drug concentration ( P<0. 05 ); correspondingly, the area under the curve of 5-FU+OX was significantly higher than those of 5-FU or OX (P<0. 05), whereas their chemosen-sitivity index, IC90 and IC50 were significantly lower than those of 5-FU or OX (P<0. 05). The chemosensitivity rates of 5-FU, OX and 5-FU+OX were 18. 5%, 14. 8% and 55. 6%, respectively; chemosensitivity of 5-FU+OX was signifi-cantly higher than that of 5-FU or OX (5-FU+OX vs. 5-FU:P=0. 001 and 5-FU+OX vs. OX:P=0. 003). Logistic re-gression analysis indicated that the chemosensitivity of 5-FU was not significantly correlated with any clinicopathological characteristics;however, the chemosensitivity of OX and 5-FU+OX was significantly correlated with Borrmann type ( OR=7. 570, P=0. 025) and tumor location (OR=3. 427, P=0. 019), respectively. Conclusion:5-FU+OX showed high in vitro chemosensitivity in gastric cancer, and may be worthy of further exploration to see its correlation with in vivo efficacy.关键词
胃癌/三磷酸腺苷/化疗敏感性/氟尿嘧啶/奥沙利铂Key words
Gastric Cancer/Adenosine Triphosphate/Chemosensitivity/Fluorouracil/Oxaliplatin分类
医药卫生引用本文复制引用
陈小东,赵平,丁志,周祥,肖硕萌,唐令超..氟尿嘧啶和奥沙利铂在胃癌中的体外化疗敏感性检测[J].肿瘤预防与治疗,2014,(4):165-170,6.基金项目
四川省科技计划项目(No.2014FZ0089)。 （No.2014FZ0089）
