山东农业大学学报（自然科学版）Issue(3)：347-351,5.DOI:10.3969/j.issn.1000-2324.2014.03.005
鸡卵巢中ANXA2基因表达调控研究
The Expression and Regulation of ANXA2 Gene in the Chicken Ovary
摘要
Abstract
ANXA2 is a family member of the membrane-bound proteins. The N-terminal region contains the tyrosine and threonine phosphorylation sites for protein kinase activation in PKC pathway regulations. Previous studies showed that ANXA2 gene is overexpressed in hens with high ovulation efficiency. This paper has studied the expression patters of ANXA2 gene during the chicken ovary and follicles development by the real-time PCR. The results showed that the expression of ANXA2 increased with the chicken ovary maturation. In hierarchy follicles, ANXA2 mRNA increased significantly as follicular development proceeds in pre-ovulatory follicles, reached the peak expression at the time of ovulation then decreased significantly after ovulation. Then, the theca cells of pre-ovulatory follicles were isolated, and treated with different concentrations of FSH or LH. We found that the theca cell ANXA2 expression was stimulated by FSH but not LH. The molecular mechanism of the ANXA2 regulation in chicken ovary should be studied further.关键词
荧光定量PCR/膜联蛋白Ⅱ/鸡/卵巢Key words
Real-time PCR/ANXA2/chicken/ovary分类
农业科技引用本文复制引用
朱桂玉..鸡卵巢中ANXA2基因表达调控研究[J].山东农业大学学报（自然科学版）,2014,(3):347-351,5.基金项目
山东省自然科学基金(ZR2013CQ002) （ZR2013CQ002）
