药学研究Issue(10)：567-570,577,5.
产梓醇地黄内生菌的分离鉴定及遗传稳定性研究
Isolation and identification of endophytic bacteria produced catalpol from rehmannia and studies on genetic stability
赵影 1徐海燕 1辛国芹 1武香玉 1谷巍1
作者信息
- 1. 山东宝来利来生物工程股份有限公司，山东 泰安 271000
- 折叠
摘要
Abstract
24 strains of entophyte were isolated from Rehmannia by tissue isolation. The 2,4 - dinitrobenzene hydrazine chromogenic method were used to determing the performance of catalpa alcohol from separated strains. The strain BLCC1 -0148 with better performance production of catalpol and good genetic stability were screened and had been identified as Ba-cillus Cereus by based on physiological,biochemical characteristics and 16S rDNA sequence phylogeny analysis. The cultur-al conditions of BLCC1 - 0148 strain was optimized by the orthogonal design experiment. The optimum medium composition of fermentation conditions was as follows:glucose 0. 5% ,tryptone 0. 5% ,yeast extract 0. 75% ,NaCl 1% . The fermentation conditions included cultural temperature 37 ℃,rotational speed of rocking bed 180 rpm,fermentation time 3d. The produc-tion of catalpol reached 839 μg·mL - 1.关键词
分离鉴定/地黄/内生菌/遗传稳定性/系统发育分析Key words
Isolation and identification/Rehmannia/Endophyte/Genetic stability/Phylogenetic analysis分类
生物科学引用本文复制引用
赵影,徐海燕,辛国芹,武香玉,谷巍..产梓醇地黄内生菌的分离鉴定及遗传稳定性研究[J].药学研究,2014,(10):567-570,577,5.
