药学研究Issue(3)：132-136,5.
金属离子介导下欧前胡素和异欧前胡素与人血白蛋白的相互作用
The interaction of imPeratorin and isoimPeratorin with HSA in the Presence of metal Ions
摘要
Abstract
Objective To obtain the intensity of fluorescence quenching,binding — site number,binding constant,the influence of reaction type and its effect on the space conformation under simulated human physiological conditions. Methods The interaction of imperatorin and isoimperatorin with human serum albumin(HSA)were investigated using the fluores-cence spectroscopy and synchronous fluorescence spectroscopy in the presence of metal ions. Results Imperatorin and iso-imperatorin enhanced the fluorescence quenching of HSA in the presence of metal ions,as well as conjugation. The hydrogen bond and vander waals force were the major driving force between the imperatorin,imperatorin and HSA. Their strength of force was changed,but the type of driving force unchanged in the presence of different metal ions. For the imperatorin,the order of force strength was Mg2 + > Cu2 + > Ni2 + > Co2 + > Zn2 + > Fe3 + > Al3 + ,for isoimperatorin,the order was Cu2 + >Zn2 + > Fe3 + > Co2 + > Mg2 + > Al3 + > Ni2 + . The results of synchronous fluorescence spectroscopy indicated that the confor-mation of HSA was changed by imperatorin or isoimperatorin in the presence of metal ions. Conclusion The presence of metal ions promoted the imperatorin and isoimperatorin interact with human serum albumin.关键词
金属离子/人血白蛋白/欧前胡素/异欧前胡素/荧光光谱法/同步荧光光谱法Key words
Metal ions/Human serum albumin/Imperatorin/Isoimperatorin/Fluorescence spectroscopy/Synchronous fluorescence spectroscopy分类
医药卫生引用本文复制引用
仝月菊,郝娟,付正卿,王娟,韩冬,张爱平..金属离子介导下欧前胡素和异欧前胡素与人血白蛋白的相互作用[J].药学研究,2015,(3):132-136,5.基金项目
山西省自然科学基金资助项目（No.2010011048-1）；山西医科大学科技创新基金 （）
