山东中医药大学学报Issue(2)：154-157,4.
滋肾疏肝胶囊对焦虑模型大鼠海马区5-HT1AR表达的影响
Effect of Zishen Shugan Capsule on 5-HT1AR Expression in Hippocampus of Anxiety Rat Model
摘要
Abstract
Objective:To study the antianxiety effect and its mechanism of Zishen Shugan capsule by estab-lishing anxiety rat model. Methods:The anxiety rat model was established by uncertainty stimulation of emp-ty bottle. The 24 rats were randomly divicled into 4 groups:control group,model group,diazepam tablet group and Zishen Shugan capsule group. Except the control group,other groups were given distilled water, diazepam tablet and Zishen Shugan capsule by gavage respectively. The expressions of mRNA and protein of 5-hydroxy tryptamine 1A receptor(5-HT1AR) were respectively detected by RT-PCR and Western blotting. The data was analyzed by One-factor analysis of variance. Results:Compared with control group,the fre-quency of both aggressive behavior and grooming behavior of model group increased significantly (P<0.01), the open arm entry percent (OE%) and the open arm time percent (OT%) of elevated plus-maze test de creased significantly(P<0.01),the mRNA and protein expressions of 5-HT1AR in the hippocampus decreased significantly(P<0.01). Compared with model group,the frequency of both aggressive behavior and groom-ing behavior of Zishen Shugan capsule group decreased significantly (P<0.01),the OE% and OT% increased significantly(P<0.01),the mRNA and protein expressions of 5-HT1AR increased significantly(P<0.01). Conclu sion:Zishen Shugan capsule had significant curative effect on anxiety rat and the gene 5-HT1AR could be the drug target.关键词
滋肾疏肝胶囊/焦虑/5-羟色胺1A受体/大鼠Key words
Zishen Shugan capsule/anxiety/5-HT1AR/rat分类
医药卫生引用本文复制引用
郝志,徐凯勇..滋肾疏肝胶囊对焦虑模型大鼠海马区5-HT1AR表达的影响[J].山东中医药大学学报,2015,(2):154-157,4.基金项目
山东省中医药科技发展计划项目 （）
