山东中医药大学学报Issue(2)：160-162,3.
健脾益智胶囊对MCAO大鼠海马β-catenin表达的影响
Effects of Jianpi Yizhi Capsule on Expression of β-catenin in Hippocampus of MCAO Rats
摘要
Abstract
Objective:To explore and discuss the effects of Jianpi Yizhi capsule on gene and protein expres-sion of β-catenin in the hippocampus of MCAO rats. Methods:120 SD rats model of MCAO were randomly divided into 5 groups:normal control group,sham operation group,model group,western medicine group and Chinese medicine group. The sham operation group was give 0.9% sodium chloride solution and the Chinese medicine group and westem medicine group were given Jianpi Yizi capsule and nimodipine mixture by gav-age respectively. 7,14 and 28 days after the operation,the protein and mRNA levels of β-catenin (Notch1 and Jagged1) in hippocampus were assayed respectively by Western blotting and Real-time PCR methods. Results:7 days after the operation,the protein and mRNA expression levels of β-catenin of the Chinese medicine group were significantly higher than all the other groups(P<0.05). 14 days after the operation, the protein and mRNA expression levels of β-catenin of the Chinese medicine group were higher than nor mal control group,sham operation group and model group(P<0.05). 28 days after the operation,the protein and mRNA levels of β-catenin of the Chinese medicine group were higher than model group ,but lower than that on the 7th day and the 14th day(P<0.05). Conclusion:Jianpi Yizhi capsule can increase protein and gene expression level of β-catenin,which suggests that the Jianpi Yizhi capsule may activate the Wnt path-way to regulate the the proliferation and differentiation of neural stem cells in the dentate gyrus of hip-pocampus after ischemia.关键词
健脾益智胶囊/大脑中动脉栓塞/β-连环蛋白/Wnt信号通路/大鼠Key words
Jianpi Yizhi capsule/middle cerebral artery occlusion/β-catenin/Wnt pathway/rat分类
医药卫生引用本文复制引用
冯珂,纪立金..健脾益智胶囊对MCAO大鼠海马β-catenin表达的影响[J].山东中医药大学学报,2015,(2):160-162,3.基金项目
国家自然科学基金资助项目 （）
