山东农业科学Issue(5)：26-28,3.
大豆子叶节遗传转化受体体系的建立
Establishment of Soybean Cotyledon Node Transgenic Receptor System
摘要
Abstract
Using three soybean varieties for materials,the effects of seed disinfection method,different hormone concentrations at bud induction and rooting stages on the regeneration of soybean cotyledon node were studied to establish an efficient regeneration system for soybean genetic transformation.The results showed that the best seed disinfection method was disinfecting with 70%alcohol for 30 seconds,then HgCl2 for 7 minutes, and finally washing 3~5 times with sterile water.The optimum bud induction medium for Jinong 28 was MS+1.5 mg/L 6-BA +0.5 mg/L IBA;for Jinong 27 and Jiunong 21,MS+2.0 mg/L 6-BA+0.8 mg/L IBA was the best.During the rooting stage,the best rooting mediums for three soybean varieties were all 1/2MS +2.0 mg/L IBA.关键词
大豆/激素/丛生芽诱导/生根Key words
Soybean/Hormone/Bud induction/Rooting分类
农业科技引用本文复制引用
曲静,刘思言,姚丹,关淑艳,王丕武,孙佳鑫..大豆子叶节遗传转化受体体系的建立[J].山东农业科学,2014,(5):26-28,3.基金项目
吉林农业大学科研启动基金（201242）；吉林省科技厅科技发展计划项目（201101111）；吉林省教育厅科学技术研究资助项目（2011-41）；吉林省科技厅项目（20120215）；吉林省科技厅成果转化补助项目 （）
