山东农业科学Issue(5)：118-121,4.
基于量子点标记技术的鸡新城疫病毒sFLISA检测技术研究
Study on sFLISA Detection Method of Newcastle Disease Virus Based on Quantum Dots Labeling Technique
摘要
Abstract
A sFLISA detection method of NDV based on QDs was founded.In this method,polyclonal antibody against NDV acted as capture antibody,monoclonal antibody against NDV labeled by biotin acted as detecting antibody,and streptavidin labeled by QDs acted as fluorescent probe.This method had no cross re-action with other avian viruses,for example,EDSV (egg drop syndrome virus),IBDV (infectious bursal dis-ease virus),and ALV (avian leukemia virus).And it was more sensitive than hemagglutination test (HA). This research gave a practical method for rapid detection of NDV at early clinical diagnosis.关键词
鸡新城疫病毒(NDV)/量子点/双抗夹心荧光免疫分析(sFLISA)Key words
Newcastal disease virus(NDV)/Quantum dots (QDs)/Sandwich fluorescence -linked immunosorbent assay(sFLISA)分类
农业科技引用本文复制引用
房保海,孙涛,贾俊涛,岳志芹,姜英辉,赵玉然,梁成珠,徐彪..基于量子点标记技术的鸡新城疫病毒sFLISA检测技术研究[J].山东农业科学,2014,(5):118-121,4.基金项目
国家质检总局科技项目“基于量子点标记技术的食源性致病菌快速检测技术研究” （）
