山东农业科学Issue(6)：1-5,5.
小麦 Clp 蛋白酶基因（TaClpP）的克隆与序列分析
Cloning and Sequence Analysis of Clp Protease Gene (TaClpP) from Wheat (Triticum aestivum L.)
摘要
Abstract
Clp protease ( ClpP) widely exists in various prokaryotes and eukaryotes , and plays important roles in the regulation of protein metabolism .In this study , a TaClpP gene ( GenBank No .KJ541961 ) was cloned from wheat ( Triticum aestivum L.) leaves by homology cloning strategy and RACE method .The cDNA sequence analysis showed that TaClpP had a single open reading frame of 897 bp; the predicted protein had 298 amino acids and the predicted molecular mass was 32 .6 kD;the isoelectric point was 9 .79 .This predic-ted protein contained a conserved S14-ClpP-2 domain motif, and had no signal peptide in its N -terminus. The supposed subcellular location of TaClpP was in chloroplast .Compared with other plants ’ ClpP members, TaClpP had high homology with its counterpart of Aegilops tauschii and Triticum urartu.In addition, the TaClpP genome sequence was obtained by PCR amplification , which contained 9 exons and 8 introns, and the length was 3 256 bp.关键词
小麦/Clp蛋白酶基因/基因克隆/序列分析Key words
Triticum aestivum L./Clp protease gene/Gene cloning/Sequence analysis分类
生物科学引用本文复制引用
张斌,杨连群,边斐,李娜娜,范仲学,宫永超,蒲艳艳,丁汉凤,彭振英..小麦 Clp 蛋白酶基因（TaClpP）的克隆与序列分析[J].山东农业科学,2014,(6):1-5,5.基金项目
国家重大科技专项转基因新品种培育“解析双特异性蛋白激酶介导的信号传递网络在提高植物抗性中的分子作用机制”（2014ZX0800916B-003）；“十二五”农村领域国家科技计划课题“小麦种质资源发掘与创新利用”（2013BAD01B02-13）；山东省科技发展计划“山东省近六十年主推小麦品种磷效率演变及磷高效品种的筛选与利用” （）
