山东农业科学Issue(6)：6-10,5.
棉花种子特异性启动子的克隆及序列分析
Cloning and Sequence Analysis of Seed-Specific Promoter in Cotton
摘要
Abstract
Promoter is a crucial cis-regulatory element of gene expression .In genetic engineering , seed-specific promoter plays an important role in regulating the specific expression of exogenous genes in seeds , im-proving the expression efficiency and enhancing the transgenic effect .In this paper , a seed-specific promoter sequence of gene D34 was cloned from Gossypium barbadense by touch down PCR amplification with the prim-ers designed based on the D34 gene of cotton LEA protein .Sequence analysis showed that the cloned fragment was 1 384 bp in length and similar to published sequence with the similarity as 94 .87%.The cloned segment not only contained promoter basic components such as TATA box , CAAT box, but also contained seed -spe-cific promoter components such as E -box , G-box and AACA motif .The biological function of these cis -el-ements was also analyzed .关键词
棉花/种子特异性启动子/序列分析Key words
Cotton/Seed-specific promoter/Sequence analysis分类
生物科学引用本文复制引用
周佳佳,孙觅真,张素青,伊海法,邢会贤,王丽媛,宋宪亮,孙学振..棉花种子特异性启动子的克隆及序列分析[J].山东农业科学,2014,(6):6-10,5.基金项目
山东省现代农业产业技术体系项目（SDAIT-07-011-02）；山东省科技发展计划项目（2012GGB01026）；山东省自然科学基金项目（ZR2013CM005）；山东省良种工程棉花品种选育（2012-2013）和棉花种质创新项目 （）
