山东农业科学Issue(7)：11-14,4.
花生叶片 DNA 快速提取方法的优化
Optimization of Rapid DNA Extraction Methods from Peanut Leaves
摘要
Abstract
With the young leaves of peanut as experiment material , four different DNA extraction meth-ods, the high-throughput DNA extraction method, the simple SDS method, TPS method and TENP method, were compared and optimized .The results showed that , compared with the other improved methods , the im-proved TPS method had the advantages of grinding samples with no liquid nitrogen , faster extraction rate , fe-wer kinds of reagent , lower cost, rapider DNA solution rate and better DNA quality .The DNA extracted through improved TPS method could be used for SSR analysis of peanut .关键词
花生/基因组DNA/提取方法/幼叶Key words
Peanut ( Arachis hypogaea L.)/Genomic DNA/Extraction method/Young leaves分类
农业科技引用本文复制引用
王蕾,赵新涛,许梦琦,宿烽,任艳,李双铃,石延茂,袁美,王传堂..花生叶片 DNA 快速提取方法的优化[J].山东农业科学,2014,(7):11-14,4.基金项目
山东省博士基金（BS2011NY014）；留学人员科技活动择优资助项目（2011）；国家科技支撑计划（2011BA35B04）；“863”子课题 （）
